UW Biochemistry Nonsense

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MF

GCTTTTATTTCCAGCTTGCAACAAAGACATTTATGTGGACCTAGACATGAAGGGCATAAACTATAACAGC [ continued ] 
TCAGTTGCCAAGAGTGCTCAATAATGCCAAGAAAGATGCACGGATGATGTCCACTGCCACTTTTTCACGTA

MR

GCTCGAATTCTTTCATCGACCACTCGAATGTCCTGGGACTCACTCACCGATGCTCCAGGCTGGGAAACTGC [ continued ] 
CTTGTGGCGTACGTGAAAAAGTGGCAGTGGACATCATCCGTGCATCTTTCTTGGCATTATTGAGCACTCTTGGCAACTGAGC


>gi|180342|gb|M18299.1|HUMCFXI06 Human coagulation factor XI gene, exon 5, clone pTZ18R
          Length = 515

                                                                       
Query: 1   gcttttatttccagcttgcaacaaagacatttatgtggacctagacatgaagggcataaa 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 34  gcttttatttccagcttgcaacaaagacatttatgtggacctagacatgaagggcataaa 93
					       
                                                                       
Query: 61  ctataacagctcagttgccaagagtgctcaataatgccaagaaagatgcacggatgatgt 120
           ||||||||||||||||||||||||||||||| ||||||||||||||||||||||||| ||
Sbjct: 94  ctataacagctcagttgccaagagtgctcaagaatgccaagaaagatgcacggatgacgt 153

                                
Query: 121 ccactgccactttttcacgta 141 	
           |||||||||||||||||||||     	
Sbjct: 154 ccactgccactttttcacgta 174	  
					   

>gi|180342|gb|M18299.1|HUMCFXI06 Human coagulation factor XI gene, exon 5, clone pTZ18R
          Length = 515
                                                                       
Query: 11  tttcatcgaccactcgaatgtcctgggactcactcaccgatgctccaggctgggaaactg 70
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 244 tttcatcgaccactcgaatgtcctgggactcactcaccgatgctccaggctgggaaactg 185
           |                      |
       244 tttcatcgaccactcgaatgtcct 221
                                                                       
Query: 71  ccttgtggcgtacgtgaaaaagtggcagtggacatcatccgtgcatctttcttggcatta 130
           ||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||| 
Sbjct: 184 ccttgtggcgtacgtgaaaaagtggcagtggacgtcatccgtgcatctttcttggcattc 125

                                  
Query: 131 ttgagcactcttggcaactgagc 153  244 tttcatcgaccactcgaatgtcct 221
           |||||||||||||||||||||||
Sbjct: 124 ttgagcactcttggcaactgagc 102




                     /translation="MIFLYQVVHFILFTSVSGECVTQLLKDTCFEGGDITTVFTPSAK
                     YCQVVCTYHPRCLLFTFTAESPSEDPTRWFTCVLKDSVTETLPRVNRTAAISGYSFKQ
                     CSHQISACNKDIYVDLDMKGINYNSSVAKSAQECQERCTDDVHCHFFTYATRQFPSLE
                     HRNICLLKHTQTGTPTRITKLDKVVSGFSLKSCALSNLACIRDIFPNTVFADSNIDSV
                     MAPDAFVSGRICTHHPGCLFFTFFSQEWPKESQRNLCLLKTSESGLPSTRIKKSKALS
                     GFSLQSCRHSIPVFCHSSFYHDTDFLGEELDIVAAKSHEACQKLCTNAVRCQFFTYTP
                     AQASCNEGKGKCYLKLSSNGSPTKILHGRGGISGYTLRLCKMDNECTTKIKPRIVGGT
                     ASVRGEWPWQVTLHTTSPTQRHLCGGSIIGNQWILTAAHCFYGVESPKILRVYSGILN
                     QSEIKEDTSFFGVQEIIIHDQYKMAESGYDIALLKLETTVNYTDSQRPICLPSKGDRN
                     VIYTDCWVTGWGYRKLRDKIQNTLQKAKIPLVTNEECQKRYRGHKITHKMICAGYREG
                     GKDACKGDSGGPLSCKHNEVWHLVGITSWGEGCAQRERPGVYTNVVEYVDWILEKTQA
                     V"
BASE COUNT      161 a    106 c     96 g    152 t
ORIGIN      About 1 kb after segment 5.
        1 gcccctagaa tctggaaggt actcatgtct tctgctttta tttccagctt gcaacaaaga
       61 catttatgtg gacctagaca tgaagggcat aaactataac agctcagttg ccaagagtgc
      121 tcaagaatgc caagaaagat gcacggatga cgtccactgc cactttttca cgtacgccac
      181 aaggcagttt cccagcctgg agcatcggtg agtgagtccc aggacattcg agtggtcgat
      241 gaaa*aacaga atcgtgattt actaaaaagc ttttgccatc aactttatgc cagaatttat
      301 tttgaacccc taaaagacat ttctataaaa gtactcctag ttttcttcat gaaaaataca
      361 cttaaagcct aatttggatg catttcattt atggtaagga gtctatcttt taataacact
      421 gtcagaaaaa tatatatact tggctaattt caaaagcgct acacttttaa attggcactt
      481 ttgaaacagc tgcaattggt atgattgtca gtgcc


5A PCR Primer 5'--GCCCCTAGAATCTGGAAGGTACTC--3'

5B PCR Primer 5'--TTTCATCGACCACTCGAATGTCCT--3'

>gi|180342|gb|M18299.1|HUMCFXI06 Human coagulation factor XI gene, exon 5, clone pTZ18R
          Length = 515

 Score = 48.1 bits (24), Expect = 1e-04
 Identities = 24/24 (100%)
 Strand = Plus / Plus

                                  
Query: 1  gcccctagaatctggaaggtactc 24
          ||||||||||||||||||||||||
Sbjct: 1  gcccctagaatctggaaggtactc 24


>gi|180342|gb|M18299.1|HUMCFXI06 Human coagulation factor XI gene, exon 5, clone pTZ18R
          Length = 515

 Score = 48.1 bits (24), Expect = 1e-04
 Identities = 24/24 (100%)
 Strand = Plus / Minus

                                   
Query: 1   tttcatcgaccactcgaatgtcct 24
           ||||||||||||||||||||||||
Sbjct: 244 tttcatcgaccactcgaatgtcct 221